Isolation and characterization of endophytic bacteria from soybean (Glycine max L.)
نویسندگان
چکیده
منابع مشابه
Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties.
A total of 276 endophytic bacteria were isolated from the root nodules of soybean (Glycine max L.) grown in 14 sites in Henan Province, China. The inhibitory activity of these bacteria against pathogenic fungus Phytophthora sojae 01 was screened in vitro. Six strains with more than 63% inhibitory activities were further characterized through optical epifluorescence microscopic observation, sequ...
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A lectin has been isolated from the roots of 5-day soybean (Glycine max (L) cultivar Chippewa) seedlings, and its properties have been compared to those of the soybean seed lectin. The sugar-binding activities of the two lectins, both in terms of specific hemagglutinating activity and sugar specificity, are indistinguishable. Molecular properties of the two lectins, measured as relative molecul...
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In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...
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A fatty acid hydroperoxidelyase (HPL) enzyme from soybean (Glycine max) seeds was purified by ammonium sulphate precipitation followed by ion exchange chromatography on DEAE-Cellulose and DEAE-Sephacel. HPL showed specific activity of 161U/mg with fold purification of 16.4 and enzyme yield was found to be 9.06%. The purified enzyme on SDSPAGE showed a prominent band of ~53KDa. Kinetic studies w...
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Seed health testing by studying the seed-borne fungi of soybean seeds is an important step in the management of the seed-borne diseases. In the present study seed-borne fungi was studied by using agar plate method. A number of seed-borne fungal species was isolated from the agar plate. These seed-borne fungal sps. were Aspergillus niger, A. flavus, A. fumigatus, Chaetomium globosum, Curvularia ...
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ژورنال
عنوان ژورنال: Journal of Central European Agriculture
سال: 2020
ISSN: 1332-9049
DOI: 10.5513/jcea01/21.1.2525